Dr Rhiannon 'Non' Evans

My research interests are in using molecular biology and physical chemistry techniques to interrogate biological catalytic systems. Additionally I have a strong background in raising public awareness of the importance of science through outreach, public engagements and publications.

rhiannon.evans@chem.ox.ac.uk

Current Position

August 2019-	Post-doctoral Research Associate Vincent Group, Department of Chemistry, University of Oxford
Jan 2014-present	Wolfson College, University of Oxford: Junior Research Fellow and College advisor
Previous Employment
April 2010 - August 2019	Post-doctoral Research Associate Armstrong Group, Department of Chemistry, University of Oxford
Education and Continued Professional Development
June 2018	Royal Society of Chemistry, Chartered Chemist status
October 2005 - Jan 2010	Postgraduate, Cardiff University, School of Chemistry (Prof R. K. Allemann): PhD Thesis Title: "Exploring the extreme - catalysis by a cold adapted dihydrofolate reductase"
Sept 2003 - Sept 2004	Biomedical Scientist (Health Professions Council Certificate of Competency), Cardiff and Vale NHS Trust, University Hospital of Wales, Department of Haematology: BSc Thesis Title: "The incidental finding of haemoglobin variants through HbA1C testing"
Sept 2001 - July "005	Undergraduate, University of Wales Institute Cardiff, BSc Biomedical Science, 1st class (Hons)
Selected Public Engagement and Outreach Events
	The Bacterial World - exhibition at the Natural History Museum, Oxford (Oct 2018 to May 2019, see here and here) Soapbox science, Cardiff - Volunteer (June 2016) 'Looking Forward - Energy and the Environment' GCSE Chemistry Workshop leader, Oxford (June 2017) Wolfson College, Oxford, President's Seminar - 'Design' (June 2016) Lead fund raiser/organiser (>£30,000 including competitive public activity grants) for 'Solving the Energy Crisis - From Ancient to Future Solar Fuels' at the Royal Society Summer Science Exhibition (2013) Chemistry Careers Day 2013, Oxford Department of Chemistry, Careers Service and Learning Institute International Year of Chemistry 2011 'Chemistry at the Garden' exhibition, University of Oxford
Funding and Awards
	Wolfson College, University of Oxford, Academic Award, Michaelmas Term, 2018 Biochemical Society independent meeting award (£500) Bioenergetics, December 2016 International Solar Fuels Young Conference, runner up, best poster presentation, 2015 Solar Fuels Network Travel Bursary (£500) International Solar Fuels Conference, 2015 University of Oxford MPLS Division Recognition Award for outstanding contribution to outreach, 2014 Royal Society of Chemistry Public Activities Grant 2013 (£5000) Biochemical Society Scientific Outreach Grant 2013 (£1000) Public engagement and outreach funding from EPSRC, 2013 (£3000) and BBSRC, 2013 (£3000) Public engagement and outreach funding from BP (British Petroleum) 2013 (£5000) Gordon Research Conference (Fe-S enzymes) best speaker, 2012 EPSRC scholarship funding (PhD Chemistry) at Cardiff University, School of Chemistry Institute of Biomedical Science Presidents Prize, Student of the Year, 2005
Publications
	28 S. Kuss, R. A. S. Couto, R. M. Evans, H. Lavender, C. C. Tang, and R. G. Compton. Versatile Electrochemical Sensing Platform for Bacteria. Analytical Chemistry Accepted February 2019 (2019) 91, 4317-4322 27 R. M. Evans, B. Siritanaratkul, C. F. Megarity, K. Pandey, T. F. Esterle, S. Badiani, and F. A. Armstrong. The value of enzymes in solar fuels research - efficient electrocatalysts through evolution. Chem. Soc. Rev. (2019) 48, 2039-2052 26 R. M. Evans* and S. E. Beaton. A novel overproduction system for the structural determination of a proton pumping [NiFe]-hydrogenase. Methods in Enzymology (2018) 613, 91-116 24 R. M. Evans, P. A. Ash, S. E. Beaton, E. J. Broke, K. A. Vincent, S. B. Carr, and F. A. Armstrong. Mechanistic exploitation of a self-repairing blocked proton transfer pathway in an O2-tolerant [NiFe] hydrogenase. J.Am.Chem.Soc. (2018) 140, 10208-10220 23 S. E. Beaton, R. M. Evans, A. J. Finney, C. M. Lamont, F. A. Armstrong, F. Sargent and S. B. Carr. The Structure of Hydrogenase-2 from Escherichia coli: Implications for H2-driven Proton Pumping. Biochemical Journal (2018) 475, 1653-1370 22 F. A. Armstrong, R. M. Evans and C. F. Megarity. Protein Film Electrochemistry of Iron-Sulfur Enzymes. Methods in Enzymology. (2018) 599, 387-407 21 P. A. Ash, S. B. Carr, H. A. Reeve, A. Skorupskaite, J. Rowbotham, R. Shutt, M. Frogley, R. M. Evans, G. Cinque, F. A. Armstrong, K. A. Vincent. Generating single metalloprotein crystals in well-defined redox states: electrochemical control combined with infrared imaging of a NiFe hydrogenase crystal. Chem.Commun. (2017) 53, 5858-5861 20 E. J. Brooke, R. M. Evans, S. T. A. Islam, G. M. Roberts, S. A. M. Wehlin, S. B. Carr, S. E. V. Phillips and F. A. Armstrong. Importance of the Active Site 'Canopy' Residues in an O2-tolerant [NiFe]-hydrogenase. Biochemistry (2017), 56, 132-142 19 F. A. Armstrong, R. M. Evans, S. V. Hexter, B. J. Murphy, M. M. Roessler, and P. Wulff. Guiding Principles of Hydrogenase Catalysis Instigated and Clarified by Protein Film Electrochemistry. Acc. Chem. Res. (2016) 49, 884-892 18 S. B. Carr, R. M. Evans, E. J. Brooke, S. A. M. Wehlin, E. Nomerotskaia, F. Sargent, F. A. Armstrong and S. E. V. Phillips. Hydrogen activation by [NiFe]-hydrogenases. Biochem.Soc.Trans (2016), 44, 863-868 17 R. M. Evans, E. J. Brooke, S. A. M. Wehlin, E. Nomerotskaia, F. Sargent, S. B. Carr, S. E. V. Phillips and F. A. Armstrong. Mechanism of Hydrogen Activation by [NiFe]-hydrogenases. Nat.Chem.Biol. (2016), 12, 46-50 16 B. J. Murphy, R. Hidalgo, M. M. Roessler, R. M. Evans, P. A. Ash, W. K. Myers, K. A. Vincent and F. A. Armstrong. Discovery of dark pH-dependent H+ migration in a [NiFe]-hydrogenase and its Mechanistic Relevance: Mobilizing the Metal-bound Hydrido Intermediate. J. Am. Chem. Soc. (2015), 137, 8484-8489 15 E. M. Behiry, R. M. Evans, J. Guo, E. J. Loveridge and R. K. Allemann. Loop interactions during catalysis by dihydrofolate reductase from M. profunda. Biochemistry (2014), 53, 4769-4774 14 R. M. Evans. Artificial photosynthesis - from ancient to future solar fuels. Catalyst: Secondary Science Review (2014) 24 (4), 6-8. https://www.stem.org.uk/elibrary/resource/34844 13 R. M. Evans and F. A. Armstrong (2014) Electrochemistry of Metalloproteins: Protein Film Electrochemistry for the Study of E. coli [NiFe]-Hydrogenase-1. Metalloproteins - Methods and Protocols. J. C. Fontecilla-Camps and Y. Nicolet (Eds) New York, Humana Press, pp., 73-94 12 R. M. Evans, A. Parkin, M. M. Roessler, B. J. Murphy, H. Adamson, M. J. Lukey, F. Sargent, A. Volbeda, J. C. Fontecilla-Camps and F. A. Armstrong. The principles of sustained enzymatic hydrogen oxidation in the presence of oxygen - the crucial influence of high potential Fe-S clusters in the electron relay of [NiFe]-hydrogenases. J. Am. Chem. Soc. (2013) 135, 2694-2707 11 E. J. Loveridge, S. M. Matthews, C. Williams, S. B.-M. Whittaker, U. L. GÜnther, R. M. Evans, W. M. Dawson, M. P. Crump and R. K. Allemann. Aliphatic 1H, 13C and 15N Chemical Shift Assignments of Dihydrofolate Reductase from the Psychropiezophile Moritella profunda in Complex with NADP+ and Folate. Biomol. NMR. Assign. (2013) 7, 61-64 10 M. M. Roessler, R. M. Evans, R. A. Davies, J. Harmer and F. A. Armstrong. EPR spectroscopic studies of the Fe-S clusters in the O2-tolerant [NiFe]-hydrogenase Hyd-1 from E. coli, and characterization of the unique [4Fe-3S] cluster by HYSCORE. J.Am.Chem.Soc. (2012) 134, 15581-94 9 E. J. Loveridge, L-H Tey, E. M. Behiry, W. M. Dawson, R. M. Evans, S. Whittaker, U. GÜnther, C. Williams, M. Crump, and R. K. Allemann. The role of large-scale motions in catalysis by dihydrofolate reductase. J.Am.Chem.Soc. (2011) 133, 20561-70 8 E. J. Loveridge, W. M. Dawson, R. M. Evans, A. Sobolewska and R. K. Allemann. Reduced susceptibility of Moritella profunda dihydrofolate reductase to trimethoprim is not due to glutamate 28. Protein.J. (2011) 30, 546-8 7 M. J. Lukey, M. M. Roessler, A. Parkin, R. M. Evans, R. A. Davies, O. Lenz, B. Friedrich, F. Sargent and F. A. Armstrong. Oxygen Tolerant [NiFe]-Hydrogenases: the Individual and Collective Importance of Supernumerary Cysteines at the Proximal Fe S Cluster. J.Am.Chem.Soc. (2011) 133, 16881-2 6 R. M. Evans, E. M. Behiry, L-H Tey, J. Guo, E. J. Loveridge, R. K. Allemann. Catalysis by dihydrofolate reductase from the psychropiezophile Moritella profunda. ChemBioChem (2010) 11, 2010-7 5 S. Hay, R. M. Evans, C. Levy, E. J. Loveridge, X. Wang, D. Leys, R. K. Allemann, and N. S. Scrutton. Are the catalytic properties of enzymes from piezophilic organisms pressure adapted? ChemBioChem. (2009) 10, 2348-53 4 R. K. Allemann, R. M. Evans, and E. J. Loveridge. Probing coupled motions in enzymatic hydrogen tunnelling reactions. Biochem.Soc.T. (2009) 37, 349-53 3 E. J. Loveridge, R. M. Evans and R. K. Allemann. Solvent effects on environmentally coupled hydrogen tunnelling during catalysis by dihydrofolate reductase from Thermotoga maritima. Chemistry - A European Journal (2008) 14, 10782-8 2 S. Hay, J. Pang, P.J. Monaghan, X. Wang, R. M. Evans, M. J. Sutcliffe, R. K. Allemann and N. S. Scrutton. Secondary kinetic isotope effects as probes of environmentally-coupled enzymatic hydrogen tunneling reactions ChemPhysChem (2008) 9, 1536-9 1 R. K. Allemann, R. M. Evans, L-H. Tey, G. Maglia, J. Pang, R. Rodriguez, P. J. Shrimpton and R. S. Swanwick. Protein motions during catalysis by dihydrofolate reductases Phil.Trans.R.Soc.B (2006) 361, 1317-21.
Selected Presentations
	Jan 2018: Gordon Research Conference: Metals in Biology - Poster presenter Dec 2016: Bioenergetics Christmas Meeting - One day symposium - Symposium Organiser Jan 2016: Gordon Research Conference: Metals in Biology - Poster presenter June 2015: Gordon Research Conference: Photosynthesis, Bentley University, USA - Invited speaker April 2015: International Solar Fuels Conference, Uppsala, Sweden - Poster presenter and Co-author Jan 2014: Frontiers Chemistry, Nottingham University, School of Chemistry - Invited speaker July 2013: 10th International Hydrogenase Conference, Szeged, Hungary - Poster presenter June 2012: Gordon Research Conference: FeS Enzymes, Mount Holyoke, USA - Invited speaker March 2012: Metal Hydrides in Biology, University of Oxford, UK - Conference organiser