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The Team

This research has been conducted within the Vincent Group at the University of Oxford and is supported by Oxford University Innovation and an Industrial Advisory Board. The Vincent Group have extensive expertise in fundamental understanding of metal-containing enzymes - this knowledge, along with the analytical and catalysis methodologies established on the project, has underpinned the development of the HydRegen technology.

Kylie Vincent Principal Investigator			Holly Reeve Project Manager / Technology & IP strategy
Miguel Ramirez Enzyme Scale up			Jack Rowbotham Heterogeneous biocataylst development
Sarah Cleary Scale up specialist			Kouji Urata New technologies
Michele Ruggeri Flow specialist			Adrian Hery Barranco Novel process design

Awards

The HydRegen Technology was the Overall Winner at the RSC Emerging Technology Competition on the 5th June 2013.

Kylie was the 'Science and Technology Woman of the Future' in 2011.

Funding

2021 Spinout funding - Innovate UK and UCSF
HydRegen has been awarded funding to form a company and spin out from the University of Oxford. The Company was incorporated in Dec 2020 and expects to start R&D in March 2021.

2020 ICURe Scheme - Holly Reeve is Entrepreneurial Lead
The HydRegen pre-spin out has been awarded a place on the ICURe scheme to carry out market validation research. This is led by Dr Holly Reeve.

2020 BIA PULSE programme - Holly Reeve selected
Holly was selected for the intensive BIA PULSE leadership and entreprenuership training programme developed by the BIA and the Francis Crick Institute, aimed at CEOs and future CEOs of companies in the life sciences.

2019 iCASE studentship
Charlotte Hancox joined the team to begin her DPhil; her research project is funded by a BBSRC iCASE Studentship through the Interdisciplinary Bioscience DTP, with support from Johnson Matthey.

2017 Public Engagement with Research Funding
Holly has been awarded funding by the MPLS division to support public engagement activties . We will develop a series of games, talks and experiments to take to secondary schools and build an online platform to inspire and inform school students about Industrial Biotechnology and the HydRegen technology.

2017 iCASE Studentship
Barnabas Poznansky joined the team to begin his DPhil; his research project looks at operating the enzyme-modified particle system in continuous flow. This is funded by an iCASE studentship through the BBSRC Interdisciplinary Bioscience DTP, with support from Dr Reddy's.

2016 IB Catalyst funding
The Vincent group have received major funding from EPSRC via Innovate UK / EPSRC / BBSRC Industrial Biotechnology Catalyst Round 3 (EP/N013514/1). The project, developing H₂-driven enzyme-catalysed chemical synthesis, started in Jan 2016 with Dr Holly Reeve as Project Manager.

2015 iCASE studentship
Michalis Posidias joined the team to begin his DPhil, his research project looks at operating the enzyme-modified particle system in the reverse direction for H⁺-driven NAD⁺ recycling to support terminal alcohol oxidation to generate aldehydes. This is funded by a BBSRC iCASE studentship with support from Johnson Matthey Catalysis and Chiral Technologies.

2014 Business Interaction Voucher
Work to extend the H₂-driven cofactor recycling system to NADPH recycling for NADPH-dependent enzymes was supported by a BBSRC Metals in Biology NIBB Business Interaction Voucher with GSK as collaborator.

2012 ERC Proof of Concept funding
R&D on this innovation was supported by ERC Proof of Concept Grant 297503 during 2012.

Publications

Front Cover Article in ACS Catalysis: J.S. Rowbotham, H.A. Reeve, K.A. Vincent, 'Hybrid Chemo-, Bio-, and Electrocatalysis for Atom-Efficient Deuteration of Cofactors in Heavy Water', ACS Catalysis, 2021, 11, 2596-2604, DOI: 110.1021/acscatal.0c03437

J.S. Rowbotham, A.P. Hardy, H.A. Reeve, K.A. Vincent, 'Synthesis of [4S-²H]NADH, [4R-²H]NADH, [4-²H₂]NADH and [4-²H]NAD⁺ cofactors through heterogeneous biocatalysis in heavy water', J Label Compd Radiopharm., 2021, 1-6, DOI: 10.1002/jlcr.3899

Preprint: Zhao, X., Cleary, S.E., Zor, C., Grobert, N., Reeve, H.A., Vincent, K.A., 'Chemo-Bio Catalysis Using Carbon Supports: Application in H₂-Driven Cofactor Recycling', ChemRxiv, 2020, Link

Preprint: Poznansky, B., Thompson, L.A., Reeve, H.A., Vincent, K.A., 'Accelerating Biocatalytic Hydrogenations using the H-Cube Flow Reactor', ChemRxiv, 2020, Link

Preprint: Srinivasan, S.J., Cleary, S.E., Paul, E.P., Ramirez, M.A., Vincent, K.A., 'E. coli Nickel-Iron Hydrogenase 1 Catalyses Non-native Reduction of Flavins: Demonstration for Alkene Hydrogenation by Old Yellow Enzyme', ChemRxiv, Link

Preissler, J., Reeve, H.A., Zhu, T., Nicholson, J., Urata, K., Lauterbach, L., Wong, L.L., Vincent, K.A., Lenz, O., 'Dihydrogen-driven NADPH recycling in imine reduction and P450-catalyzed oxidations mediated by an engineered O₂-tolerant hydrogenase', ChemCatChem, 2020, 12, 4835-4851, DOI: 10.1002/cctc.202000763

Thompson, L.A., Rowbotham, J.S., Nicholson, J.H., Ramirez, M.A., Zor, C., Reeve, H.A., Grobert, N., Vincent, K.A., 'Rapid, Heterogeneous Biocatalytic Hydrogenation and Deuteration in a Continuous Flow Reactor', ChemCatChem, 2020, 12, 3913-3918, DOI: 10.1002/cctc.202000161

J.S. Rowbotham, M.A. Ramirez, O. Lenz, H.A. Reeve, K.A. Vincent, 'Bringing biocatalytic deuteration into the toolbox of asymmetric isotopic labelling techniques', Nature Communications, 2020, 11, 1454, DOI: 10.1038/s41467-020-15310-z

B. Poznansky, L.A. Thompson, S.A. Warren, H.A. Reeve, K.A. Vincent, 'Carbon as a Simple Support for Redox Biocatalysis in Continuous Flow', Org. Process Res. Dev., 2020, Special Issure: Flow Chemistry Enabling Efficient Synthesis. DOI: 10.1021/acs.oprd.9b00410

Thompson, L.A., Rowbotham, J.S., Reeve, H.A., Zor, Z., Grobert, N., Vincent, K.A., Chapter 18: Biocatalytic Hydrogenations on Carbon Supports in 'Nanoarchitectures Built with Carbon Nanotubes and Magnetic Nanoparticles', Methods in Enzymology, Editor - Kumar, 2020, Vol. 630, pp303-325. DOI: 10.1016/bs.mie.2019.10.017

Preprint: Rowbotham, J.S., Lenz, O., Reeve, H.A., Vincent, K.A., 2019, ChemRxiv DOI: https://doi.org/10.26434/chemrxiv.7982864.v1

Cover article in ChemCommun: Zor, C., Reeve, H.A., Quinson, J., Thompson, L.A., Lonsdale, T.H., Dillon, F., Grobert, N., Vincent K.A., 'H₂-driven Biocatalytic Hydrogenation in Continuous Flow using Enzyme-Modified Carbon Nanotube Columns', Chem. Commun., 2017, 53, 9839-9841 DOI:10.1039/C7CC04465H

Cover Article in Biochemical Journal: Reeve, H.A., Ash, P.A., Park, H., Huang, A., Posidias, M., Tomlinson, C., Lenz, O., Vincent, K.A., 'Enzymes as modular catalysts for redox half reactions in H₂-powered chemical synthesis: from biology to technology', Biochemical Journal, 2017, 474, 215-230 DOI:10.1042/BCJ20160513

Cover Article in ChemCatChem: Reeve, H.A., Lauterbach, L., Lenz, O., Vincent, K.A. 'Enzyme-Modified Particles for Selective Bio-Catalytic Hydrogenation via H₂-driven NADH Recycling' ChemCatChem, 2015, 7, 21, 3480 - 3487 DOI:10.1002/cctc.201500766

Reeve, H.A., Lauterbach, L., Ash, P.A., Lenz, O., Vincent, K.A., 'A modular system for regeneration of NAD cofactors using graphite particles modified with hydrogenase and diaphorase moieties' Chem. Commun. 2012, 48 (10), 1589-1591. Link.

Lauterbach, L., Idris, Z., Vincent, K.A., Lenz, O. 'Catalytic properties of the isolated diaphorase fragment of the NAD⁺-reducing [NiFe]-hydrogenase from Ralstonia eutropha' PLoS ONE, 2011, 6, (10): e25939. Link.

Intellectual Property

A patent covering the HydRegen technology was filed in 2011 and is granted in the EU and in the final stages of consideration in the US. Publication number: WO2013050760 A2.
More information about the technology can be found on the Oxford University Innovation website.

Two further patent applications have been filed through OUI, with further IP protection in progress.