Kylie Vincent's Group
Department of Chemistry,
University of Oxford,
Inorganic Chemistry Laboratory,
South Parks Road, OX1 3QR
kylie.vincent@chem.ox.ac.uk
Animation about Industrial Biotechnology and HydRegen
Video: Unlocking the Power of Hydrogen
ABC Radio Science Show: Cleaner greener method for making lab chemicals
Podcast: Learning from nature
Women in Science: Cleaner chemicals
Video: Chemistry in the garden
Chemistry World Video: about the HydRegen technology

Publications

Ash, P.A., Carr, S.B., Reeve, H.A., Skorupskaite, A., Rowbotham, J., Shutt, R., Frogley, M., Evans, R.M., Cinque, G., Armstrong, F.A., Vincent, K.A., 'A method for generating single metalloprotein crystals in well-defined redox states: combined electrochemical control and infrared microspectroscopic imaging of a NiFe hydrogenase crystal', Chem. Commun., 2017, 53, 5858-5861. DOI:10.1039/C7CC02591B

Ash, P.A., Hidalgo, R., Vincent, K.A., 'Proton transfer in the catalytic cycle of NiFe hydrogenases: insight from vibrational spectroscopy', ACS Catalysis, 2017, 7, 4, 2471-2485. DOI:10.1021/acscatal.6b03182

Paengnakorn, P., Ash, P.A., Shaw, S., Danyal, K., Chen, T., Dean, D.R., Seefeldt, L.C., Vincent, K.A., 'Infrared spectroscopy of the nitrogenase MoFe protein under electrochemical control: potential-triggered CO binding', Chemical Science, 2017, 8, 1500-1505. DOI:10.1039/C6SC02860H

Reeve, H.A., Ash, P.A., Park, H., Huang, A., Posidias, M., Tomlinson, C., Lenz, O., Vincent, K.A., 'Enzymes as modular catalysts for redox half reactions in H2-powered chemical synthesis: from biology to technology', Biochemical Journal, 2017, 474, 215-230. DOI:10.1042/BCJ20160513

Ash, P. A., Vincent, K. A., 'Vibrational Spectroscopic Techniques for Probing Bioelectrochemical Systems' in 'Biophotoelectrochemistry: From Bioelectrochemistry to Biophotovoltaics', Volume 158 of the series'Advances in Biochemical Engineering / Biotechnology', pp 75-110, Springer, 2016. DOI:10.1007/10_2016_3

McPherson, I.J., Ash, P.A., Jacobs, R.M.J., Vincent, K.A. 'Formate adsorption on Pt nanoparticles during formic acid electro-oxidation: insights from in situ infrared spectroscopy', Chem. Commun., 2016, 52, 12665-12668. DOI:10.1039/C6CC05955D

Zheng, W., Nayak, S., Yuan, W., Zeng, Z., Hong, X., Vincent, K.A., Tsang, S.C. 'A tunable metal-polyaniline interface for efficient carbon dioxide electro-reduction to formic acid and methanol in aqueous solution' Chem Commun, 2016, 52, 13901-13904. DOI:10.1039/C6CC07212G

Nayak, P.K., Moore, D.T., Wenger, B., Nayak, S., Haghighirad, A.A., Fineberg, A., Noel, N.K., Reid, O.G., Rumbles, G., Kukura, P., Vincent, K.A., Snaith, H.J. 'Mechanism for rapid growth of organic-inorganic halide perovskite crystals.' Nature Communications, 2016, 7, 13303. DOI:10.1038/ncomms13303

Ash, P.A., Reeve, H.A., Quinson, J., Hidalgo, R., Zhu, T., McPherson, I.J., Chung, M., Healy, A.J., Nayak, S., Lonsdale, T.H., Wehbe, K., Kelley, C.S., Frogley, M.D., Cinque, G., Vincent, K.A. 'Synchrotron-based Infrared Microanalysis of Biological Redox Processes under Electrochemical Control' Anal. Chem., 2016, 88, 6666-6671. DOI:10.1021/acs.analchem.6b00898

Reeve, H.A., Lauterbach, L., Lenz, O., Vincent, K.A. 'Enzyme-Modified Particles for Selective Bio-Catalytic Hydrogenation via H2-driven NADH Recycling' ChemCatChem, 2015, 7, 21, 3480-3487 DOI:10.1002/cctc.201500766

Ash, P.A., Liu, J., Coutard, N., Heidary, N., Horch, M., Gudim, I., Simler, T., Zebger, I., Lenz, O., Vincent, K.A., 'Electrochemical and Infrared Spectroscopic Studies Provide Insight into Reactions of the NiFe Regulatory Hydrogenase from Ralstonia eutropha with O2 and CO' J. Phys. Chem. B 2015, 119, 13807-13815. DOI:10.1021/acs.jpcb.5b04164

Murphy, B.J., Hidalgo, R., Roessler, M.M., Evans, R.M., Ash, P.A., Myers, W.K., Vincent, K.A., Armstrong, F.A. 'Discovery of Dark pH-Dependent H+ Migration in a [NiFe]-hydrogenase and Its Mechanistic Relevance: Mobilizing the Hydrido Ligand of the Ni-C Intermediate' J. Am. Chem. Soc. 2015, 137, 8484-8489. DOI:10.1021/jacs.5b03182

Duca, M., Weeks, J.R., Fedor, J.G., Weiner, J.H., Vincent, K.A. 'Combining Noble Metals and Enzymes for Relay Cascade Electrocatalysis of Nitrate Reduction to Ammonia at Neutral pH' ChemElectroChem 2015, 2, 1086-1089. DOI:10.1002/celc.201500166

Hidalgo, R., Ash, P.A., Healy, A.J., Vincent, K.A. 'Infrared Spectroscopy During Electrocatalytic Turnover Reveals the Ni-L Active Site State During H2 Oxidation by a NiFe Hydrogenase' Angew. Chemie. Int. Ed. 2015, 54, 7110-7113. DOI:10.1002/anie.201502338

Quinson, J., Hidalgo, R., Ash, P.A., Dillon, F., Grobert, N., Vincent, K.A. 'Comparison of carbon materials as electrodes for enzyme electrocatalysis: hydrogenase as a case study' Faraday Disc. 2014, 172, 473-496. Link

Grabarczyk, D.B., Ash, P.A., Vincent, K.A. 'Infrared Spectroscopy Provides Insight into the Role of Dioxygen in the Nitrosylation Pathway of a [2Fe2S] Cluster Iron-Sulfur Protein' J. Am. Chem. Soc., 2014, 136, 11236-11239. Link

Hexter, S.V., Chung, M.-W., Vincent, K.A., Armstrong, F.A.'Unusual Reaction of [NiFe]-Hydrogenases with Cyanide' J. Am. Chem. Soc., 2014, 136, 10470-10477. Link

McPherson, I.J., Vincent, K.A. 'Electrocatalysis by hydrogenases: lessons for building bio-inspired devices' J. Braz. Chem. Soc., 2014, 25, 427-441. Link

Healy, A.J., Ash, P.A., Lenz, O., Vincent, K.A. 'Attenuated total reflectance infrared spectroelectrochemistry at a carbon particle electrode; unmediated redox control of a [NiFe]-hydrogenase solution' Phys Chem Chem Phys, 2013, 15, 7055-7059. Link

Lauterbach, L., Lenz, O., Vincent, K.A. 'H2-driven cofactor regeneration using NAD(P)+-reducing hydrogenases' FEBs Journal, 2013, 280, 3058-3068. Link

Reeve, H.A., Vincent, K.A., 'Bioelectrocatalysis for Direct Alcohol Fuel Cells' in Z.-X. Liang and T.S. Zhao (Eds.), Catalysts for Alcohol-Fuelled Direct Oxidation Fuel Cells, RSC, 2012. Link

Reeve, H.A., Lauterbach, L., Ash, P.A., Lenz, O., Vincent, K.A., 'A modular system for regeneration of NAD cofactors using graphite particles modified with hydrogenase and diaphorase moieties' Chem. Commun. 2012, 48 (10), 1589-1591. Link.

Ash, P.A., Vincent, K.A. 'Spectroscopic analysis of immobilised redox enzymes under direct electrochemical control' Chem. Commun., 2012, 48 (10), 1400-1409.Link.


Lauterbach, L., Idris, Z., Vincent, K.A., Lenz, O. 'Catalytic properties of the isolated diaphorase fragment of the NAD+-reducing [NiFe]-hydrogenase from Ralstonia eutropha' PLoS ONE, 2011, 6, (10): e25939. Link.

Healy, A.J., Reeve, H.A., Parkin, A., Vincent, K.A. 'Electrically conducting particle networks in polymer electrolyte as three-dimensional electrodes for hydrogenase electrocatalysis' Electrochimica Acta (2011) 56, 28, 10786-10790. Link.

Lauterbach, L., Liu, J., Horch, M., Hummel, P., Schwarze, A., Haumann, M., Vincent, K.A., Lenz, O., Zebger, I. 'The hydrogenase subcomplex of the NAD+-reducing [NiFe]-hydrogenase from Ralstonia eutropha: insights into catalysis and redox interconversions' Eur. J. Inorg. Chem., 2011, 1067-1079. Link


Healy, A.J., Reeve, H.A., Vincent, K.A. 'Development of an infrared spectroscopic approach for studying metalloenzyme active site chemistry under direct electrochemical control' Faraday Discussions. (2011) 148 345 - 357. Link

Presented at Faraday Discussion 148: Spectroscopy, Theory and Mechanism in Bioinorganic Chemistry, 2010, University of Nottingham

Lubner, C.E., Knorzer, P., Silva, P.J.N., Vincent, K.A., Happe, T., Bryant, D.A., Golbeck, J.H. 'Wiring an [FeFe]-Hydrogenase with Photosystem I for Light-Induced Hydrogen Production' Biochemistry 2010 49 10264-10266. Link

In Biochemistry's Most Read Rapid Reports of 2011

Danyal, K., Inglet, B.S., Vincent, K.A., Barney, B.M., Hoffman, B.M., Armstrong, F.A., Dean, D.R., Seefeldt, L.C. 'Uncoupling Nitrogenase: Catalytic Reduction of Hydrazine to Ammonia by a MoFe Protein in the Absence of Fe Protein-ATP' J. Am. Chem. Soc. (2010) 132 13197-13199. Link


Highlighted in a JACS Select collection focused on chemical mechanisms in biochemistry

Vincent, K.A. 'Triggered Infrared spectroscopy for investigating metalloprotein chemistry' Philosophical Transactions of the Royal Society A (2010) 368, 3713-3731. Link

Stripp, S., Goldet, G., Brandmayr, C., Sanganas, O., Vincent, K.A., Haumann, M., Armstrong, F.A., Happe, T. 'How Oxygen attacks [FeFe] Hydrogenases from photosynthetic Organisms' Proc. Natl Acad. Sci. USA (2009) 106, 17331-17336. Link

Vincent, K.A., Barton, S.C., Canters, G.W., Heering, H.A. 'Electrocatalysis for fuel cells at protein-modified electrodes' in Koper, M. (Ed.) Fuel Cell Catalysis: A Surface Science Approach, John Wiley & Sons, USA, 2009. (Book Chapter). Link

Woolerton, T.W., Vincent, K.A. 'Oxidation of dilute H2 and H2 / O2 mixtures by hydrogenases and Pt' Electrochimica Acta (2009) 54, 5011-5017. Link

Ludwig, M., Cracknell, J.A., Vincent, K.A., Armstrong, F.A., Lenz, O. 'Oxygen-Tolerant H2 Oxidation By Membrane-Bound [NiFe]-Hydrogenases of Ralstonia species: Coping with Low-Level H2 in Air' J. Biol. Chem. (2009) 284, 465-477. Link

Best, S.P., Borg, S.J., Vincent, K.A., 'Infrared Spectroelectrochemistry' in Kaim, W. and Klein, A. (Eds) Spectroelectrochemistry, Royal Society of Chemistry, 2008. (Book Chapter). Link

Armstrong, F.A., Belsey, N.A., Cracknell, J.A., Goldet, G., Parkin, A., Reisner, E., Vincent, K.A., Wait, A.F. 'Dynamic electrochemical investigations of hydrogen oxidation and production by enzymes and implications for future technology' Chem. Soc. Rev. (2009) 38, 36-51. Link

Cracknell, J.A., Vincent, K.A., Armstrong, F.A. 'Enzymes as Working or Inspirational Electrocatalysts for Fuel Cells and Electrolysis' Chem. Rev. (2008) 108, 7, 2439-2461. Link

Goldet, G., Wait, A.F., Cracknell, J.A., Vincent, K.A., Ludwig, M., Lenz, O., Friedrich, B., Armstrong, F.A. 'Hydrogen Production under Aerobic Conditions by Membrane-Bound Hydrogenases from Ralstonia Species' J. Am. Chem. Soc. (2008) 130, 33, 11106-11113. Link

Cracknell, J.A., Vincent, K.A., Ludwig, M., Lenz, O., Friedrich, B., Armstrong, F.A. 'Enzymatic Oxidation of H2 in Atmospheric O2: The Electrochemistry of Energy Generation from Trace H2 by Aerobic Microorganisms' J. Am. Chem. Soc. (2008) 130, 2, 424-425. Link

Vincent, K.A., Parkin, A., Armstrong, F.A. 'Investigating and exploiting the electrocatalytic properties of hydrogenases' Chem. Rev. (2007) 107, 10, 4366-4413. Link

Vincent, K.A., Li, X., Blanford, C.F., Belsey, N.A., Weiner, J.H., Armstrong, F.A. 'Enzymatic catalysis on conducting graphite particles' Nature Chem. Biol. (2007) 3, 12, 761-762. Link

Parkin, A. Seravalli, J., Vincent, K.A. Ragsdale, S.W., Armstrong, F.A. 'Rapid and Efficient Electrocatalytic CO2 / CO Interconversions by Carboxydothermus hydrogenoformans CO Dehydrogenase I on an Electrode' J. Am. Chem. Soc. (2007) 129, 34, 10328-10329. Link

Vincent, K.A., Cracknell, J.A., Clark, J.R., Ludwig, M., Lenz, O., Friedrich, B., Armstrong, F.A. 'Electricity from low-level H2 in still air - an ultimate test for an oxygen tolerant hydrogenase' Chem. Commun. (2006) 5033-5035. Link

Vincent, K.A., Belsey, N.A., Lubitz, W., Armstrong, F.A., 'Rapid and Reversible Reactions of [NiFe]-Hydrogenases with Sulfide.' J. Am. Chem. Soc. (2006) 128, 23, 7448-7449. Link

Vincent, K. A., Cracknell, J. A., Lenz, O., Zebger, I., Friedrich, B., Armstrong, F. A., 'Electrocatalytic hydrogen oxidation by an enzyme at high carbon monoxide or oxygen levels' Proc. Natl Acad. Sci. USA (2005) 102, 47, 16951-16954. Link

Vincent, K. A., Parkin, A., Lenz, O., Albracht, S. P. J., Fontecilla-Camps, J. C., Cammack, R., Friedrich, B., Armstrong, F. A., 'Electrochemical Definitions of O2 Sensitivity and Oxidative Inactivation in Hydrogenases' J. Am. Chem. Soc. (2005) 127, 51, 18179-18189.

Vincent, K. A., Cracknell, J. A., Parkin, A., Armstrong, F. A., 'Hydrogen cycling by enzymes: electrocatalysis and implications for future energy technology.' Dalton Trans. (2005) 21, 3397-3403.

Vincent, K. A., Armstrong, F. A., 'Investigating Metalloenzyme Reactions Using Electrochemical Sweeps and Steps: Fine Control and Measurements with Reactants Ranging from Ions to Gases' Inorg. Chem. (2005) 44, 4, 798-809.

Pickett, C. J., Vincent, K. A., Ibrahim, S. K., Gormal, C. A., Smith, B. E., Fairhurst, S. A., Best, S. P., 'Synergic binding of carbon monoxide and cyanide to the FeMo cofactor of nitrogenase: Relic chemistry of an ancient enzyme?' Chem. Eur. J. (2004) 10, 19, 4770-4776.

Lamle, S. E., Vincent, K. A., Halliwell, L. M., Albracht, S. P. J., Armstrong, F. A., 'Hydrogenase on an electrode: a remarkable heterogeneous catalyst' Dalton Trans. (2003) 21, 4152-4157.

Pickett, C. J., Vincent, K. A., Ibrahim, S. K., Gormal, C. A., Smith, B. E., Best, S. P., 'Electron-transfer chemistry of the iron-molybdenum cofactor of nitrogenase: Delocalized and localized reduced states of FeMoco which allow binding of carbon monoxide to iron and molybdenum' Chem. Eur. J. (2003) 9, 1, 76-87.

Vincent, K. A., Tilley, G. J., Quammie, N. C., Streeter, I., Burgess, B. K., Cheesman, M. R., Armstrong, F. A., 'Instantaneous, stoichiometric generation of powerfully reducing states of protein active sites using Eu(II) and polyaminocarboxylate ligands' Chem. Commun. (2003) 2590-2591.

Jones, A. K., Lamle, S. E., Pershad, H. R., Vincent, K. A., Albracht, S. P. J., Armstrong, F. A., 'Enzyme Electrokinetics: Electrochemical Studies of the Anaerobic Interconversions between Active and Inactive States of Allochromatium vinosum [NiFe]-hydrogenase' J. Am. Chem. Soc. (2003) 125, 28, 8505-8514.

Ibrahim, S. K., Vincent, K., Gormal, C. A., Smith, B. E., Best, S. P., Pickett, C. J., 'The isolated iron-molybdenum cofactor of nitrogenase binds carbon monoxide upon electrochemically accessing reduced states' Chem. Commun. (1999) 1019-1020.